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Detection of low-frequency variants with VarSome Clinical

Learn how you can detect low-frequency variants with VarSome Clinical by utilizing UMIs in your NGS workflow.

  • What are Unique Molecular Identifiers (UMI)?
  • Differing Technological Approaches
  • What does this mean for VarSome Clinical users?
UMIs

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Detection of low-frequency variants with VarSome Clinical

Download Now!

What are Unique Molecular Identifiers (UMI)?

What are Unique Molecular Identifiers (UMI)?

Unique Molecular Identifiers are short molecular tags used in some NGS library preparation protocols. The tags are added to DNA fragments prior to PCR amplification in order to detect and quantify unique DNA fragments and/or to detect low-frequency variants typically found in somatic samples.

Differing Technological Approaches

Differing Technological Approaches

When evaluating UMI options there are a variety of factors to consider in order to make the choice that fits best with the targeted sequencing technology utilized in your NGS workflow. The availability of human resources in the lab, level of automation, and throughput are important examples.

 

What does this mean for VarSome Clinical users?

What does this mean for VarSome Clinical users?

VarSome Clinical is technology agnostic and supports the data processing pipeline for assays using UMIs, both Amplicon and Hybrid Capture.